Micrographs of the leaf

Figure 33. Draw what you see! This hand drawing demonstrates that looking at a leaflet section through the microscope can also be captured by drawing the image. Trichome (black arrow), upper (adaxial) leaflet epidermis (blue arrow), palisade mesophyll (yellow arrow), spongy mesophyll (white arrow) and lower (abaxial) epidermis (red arrow). Credit: C.G. Elowsky

Figure 34. Light microscope view of a bean leaf.  A thick cross-section through a bean leaflet showing the adaxial epidermis (blue arrow), palisade mesophyll (yellow arrow), spongy mesophyll (white arrow), abaxial epidermis (red arrow) with hooked trichomes (black arrow). The green color shows the chloroplasts. This section was made from a fresh green leaf. Magnified 100 times. Credit: C.G. Elowsky

Figure 35. Higher magnification view of a cross-section of a bean leaflet. A thick cross-section through a bean leaflet showing the adaxial epidermis (blue arrow), palisade mesophyll (yellow arrow), spongy mesophyll (white arrow), abaxial epidermis (red arrow) with a trichome (black arrow). The green colors are chloroplasts. This section was made from a fresh green leaf. The star indicates an empty space where gas exchange can occur.  Magnified 200 times. Credit: C.G. Elowsky

Figure 36. Cross-section through a preserved leaflet stained with safranin and toluidine blue O. A different view of the cross-section through a bean leaflet showing the adaxial epidermis (blue arrow), palisade parenchyma (yellow arrow), spongy mesophyll (white arrow) and abaxial epidermis (red arrow). The blue discs are chloroplasts. Note the air spaces. Slide prepared by the Nebraska Veterinary Diagnostic Center. Magnified 125 times. Credit: M.E. Conley

Figure 37. A cross-section through the midvein of a preserved bean leaflet. The midvein is the big, middle vein (midrib) in a bean leaflet. This cross-section shows the adaxial epidermis (blue arrow), palisade mesophyll (yellow arrow), spongy mesophyll (white arrow), abaxial epidermis (red arrow) and the midrib (black arrow). The midrib also contains a vascular bundle which contains xylem and phloem tissues. Slide prepared by the Nebraska Veterinary Diagnostic Center. Magnified 50 times. Credit: M.E. Conley 

Figure 38. A 3-dimensional view of the main big vein (midrib). The midrib runs down the center of a leaflet directly into a stem.  This photo is of the middle leaflet of the third trifoliate leaf. Credit: E.T. Paparozzi

Figure 39. A closer view of the midrib. Note the trichomes (black arrows), adaxial epidermis (blue arrow), the xylem in the vascular bundle (yellow arrow) and the abaxial epidermis (red arrow) This is the midrib of the middle leaflet of the third trifoliate leaf. Credit: E.T. Paparozzi

Figure 40.  A high magnification view of the xylem. A high magnification of vessels (water tubes) in the midrib of the middle leaflet of the third trifoliate leaf. Credit: E.T. Paparozzi