Crop Management

DNA and DNA Extraction

This lesson discusses what DNA is and how it relates to genes and chromosomes. How and why DNA is extracted in the genetic engineering process is also covered.

Gene Design 2 - Gene Constructs

This lesson builds upon the gene region lesson discussing the gene construct of currently used hybrids and explaining how these combinations result in a particular gene expression.

Overview of Plant Genetic Engineering

A general description of the overall process of genetic engineering. A basic explanation of the five steps for genetically engineering a crop is provided.

Descripción de la Ingeniería Genética

La ingeniería genética es un tipo de modificación genética que consiste en la adición dirigida de uno o mas genes ajenos al genoma de un organismo. Un gen posee la información que dará a un organismo una característica específica.

Polymerase Chain Reaction (PCR)

The polymerase chain reaction (PCR) laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes and its basic requirements for success. Examples of interpreting results are given. PCR's strengths, weaknesses and applications to plant biotechnology are explained.

Protein Detection in Plants

This lesson will focus on molecular principles involved in the detection of biotechnology derived proteins in crops, using the lateral flow ELISA.

Real Time PCR - Some Basic Principles

Real time PCR is a laboratory technique that can perform relatively accurate, reliable and reproducible measurements, to quantitatively determine the presence of specific gene sequences. Its value is being recognized in a variety of applications, including transgenic (GMO) detection. It is becoming increasingly important to know what percentage of a particular transgene is present in an export shipment, for example. Real time PCR can also be used to support more traditional plant breeding techniques, making the process of distinguishing allelic variations more efficient. This lesson explains the principles of real time PCR and its' application, with examples in plant breeding and GMO detection.

The New Bts

Bt corn is a general term used to describe corn hybrid farmers buy that have an additional gene added to one on their chromosomes which originated from the soil bacteria Bacillus thuringiensis (Bt). This lesson tells the story of how different hybrids can contain different Bt genes which encode proteins that have toxicity to different corn pest insects.