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Real Time PCR - Some Basic Principles
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Real Time PCR - Some Basic Principles
Authors
Deana M. Namuth, Department of Agronomy and Horticulture, University of Nebraska-Lincoln
G. Ronald Jenkins, United States Department of Agriculture Grain Inspection, Packers and Stockyards Administration
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Real Time PCR - Some Basic Principles
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Real Time PCR - Some Basic Principles Introduction
Need for Detecting GMOs?
GMO Detection
Presence/Absence Not Enough
Defining Real Time PCR
Temperature Cycles in Real Time PCR
Real Time PCR Components
Probe Systems - SYBR Green
Probe Systems - FRET
Probe Systems - Taqman
Using Data from Real Time PCR
Strengths/weaknesses of Real Time PCR
Summary for Real Time PCR - Basic Principles
References for Real Time PCR - Basic Principles
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