Part 3: Transformation and Tissue Culture
Part 3: Transformation and Tissue Culture contains lessons that detail how genetic changes are intentionally produced in plants through transformation and plant tissue culture.
Theories Behind Plant Tissue Culture
When introducing a foreign gene into a target genome in plant tissue, you need to grow the transgenic cell to a complete plant. This is done by plant tissue culture, a biotechnique based on the concept that an organ, tissue or cell of a plant can be manipulated to grow back into a complete plant.
Transformation 1 - Plant Tissue Culture
This lesson explains the technique of tissue culture as used in plant transformation. It discusses important issues, such as the use of selectable markers, genotype specificity, and tissue culture alternatives.
Transformation 2 - Transformation Methods
This lesson explains the procedure of introducing a new gene into a plant cell (transformation). It discusses the main goals of the transformation process and describes the four main methods of transformation.
Transformation 3 - Transformation Events
This lesson defines an 'event'. It explains the determining factors specific to an event, the qualities of a desirable event, and the identification and selection of desirable events.