Part 2: Gene Cloning and Design | Genetics Book 2: Biotechnology Principles and Practices

Part 2: Gene Cloning and Design contains lessons on the laboratory processes associated with biotechnology, including gene cloning and design of transgenes.

Gene Cloning

This lesson covers the utilization of gene cloning to isolate and copy a specific gene of interest. The transformation of bacteria with plasmids containing antibiotic resistance genes to make gene libraries and the selection of bacteria colonies that contain the specific gene of interest are described.

Gene Cloning Part 1: The Mechanics of Recombinant DNA

This lesson describes how DNA molecules can be recombined to make recombinant DNA and how special DNA molecules called plasmids allow scientists to clone genes.

Gene Cloning Part 2: Making and Screening Gene Libraries

This lesson teaches how a specific gene can be identified from among the thousands of genes that can be cloned from an organism.

Gene Design 1 - Gene Regions

This lesson describes the three gene regions and their roles in gene expression. It also discusses how the regions of a gene can be altered to obtain desired trait expression.

Gene Design 2 - Gene Constructs

This lesson builds upon the gene region lesson discussing the gene construct of currently used hybrids and explaining how these combinations result in a particular gene expression.