Introduction for PCR
Overview:
The polymerase chain reaction laboratory technique is used in a variety of applications to make copies of a specific DNA sequence. This lesson describes how a PCR reaction works, what it accomplishes and its basic requirements for success. Examples of interpreting results are given. PCR’s strengths, weaknesses and applications to plant biotechnology are explained.
Objectives:
At the completion of this PCR lesson, learners will be able to:
-Describe what natural cellular process PCR mimics.
-List the 5 chemical components of a PCR reaction and describe their roles.
-List the functions of the 3 temperature cycles which are repeated during a PCR reaction.
-Describe the process of observing results and interpreting results of an experiment.
-Explain both the strengths and weaknesses of PCR.
-List possible uses of PCR in plant biotechnology.
Development of this lesson was supported in part by Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 98-EATP-1-0403 administered by Cornell University and the American Distance Education Consortium (ADEC); and in part by USDA Initiative for Future Agriculture and Food Systems (IFAFS)and the Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number 00-52100-9710. A contribution of the University of Nebraska College of Agricultural Sciences and Natural Resources, Lincoln NE 68583, Journal Series 04-12. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.