Drought

How to use this resource

This scenario accompanies the online lesson, "Transpiration - Water Movement Through Plants", and is designed to allow you to apply the concepts learned in that lesson to a real-life problem.

Meet Brandon, a farmer in south-central Nebraska. Brandon raises daughters for fun and corn for profit.

Brandon and family in the field.  Credit:  Lisa Hunnicutt

Brandon hears about a new type of genetically engineered corn that is being developed to be drought resistant. Brandon is an expert on growing corn in water limiting conditions.

Quiz

Question

Question: Why would limited water be important in growing corn?

To review this concept click on the link: Transpiration - What and Why?

Looks Good! Correct: Water does all of these for the plant.

Brandon has learned about the crop genetic engineering process and knows that scientists can add a single gene to a plant like corn and change a single trait in the plant. Biotechnology companies are doing this to develop new types of corn for farmers to grow. Companies, however, will not reveal all the details of their new projects until they are finished testing them. Learn about the plant genetic engineering processes in Overview of Plant Genetic Engineering: Overview and Objectives.

Crop genetic engineering includes: 1) DNA isolation 2) gene cloning 3) gene design 4) transformation 5) plant breeding

Brandon wonders if adding a single gene to corn can affect a trait like drought resistance. Brandon believes that his success in farming depends on his ability to think ahead about the practical value of different options. Therefore, he contacts his teacher friends in the Agronomy and Horticulture department. He wants to know what they can tell him about this drought resistance trait in corn.

One teacher and researcher in Agronomy and Horticulture is Dr. Jim Specht. Jim is an expert in soybeans and how they use their genes to grow under water limited conditions. He has a pretty good idea what the biotechnology company is doing to produce their drought resistant genetically engineered plants.

IANR Soybean Geneticist Jim Specht examines soybean DNA patterns in his lab.  Credit: Research Nebraska, March 2001

The gene the biotechnology company has inserted into corn is probably a version of the DREB1A gene. The DREB1A activates the expression of other genes that govern the plant’s response to cellular dehydration and when these genes are turned on, they produce proteins that allows the plant to better cope with water stress. Jim’s hypothesis is that this work involved some very good molecular genetics research. However, Jim believes that in growing conditions in places like Nebraska, the DREB gene would ultimately influence the function of the cells pictured below.

Leaf surface cells. Credit: Ellen Paparozzi

Quiz

Question

Question: What are these paired cells called that are found on the surface of leaf cells?

Looks Good! Correct: Paired guard cells can work together to form an opening on the leaf surface.
Question
Stomate side view

Question: What is the open pore called that is formed when the paired cells fill with water?

 

Looks Good! Correct: Stomata or stomates are the opening formed by guard cells.
Question
Stomate side view

Question: How are stomates related to the water status of the plant?

Looks Good! Correct: Water leaves the plant as a vapor or gas.
Question
Stomate side view

Question: What factors influence stomata function?

Looks Good! Correct: Many factor acn influence stomata function.

The biotechnology company shows pictures of plants with the transgene (genetically engineered plants). They do not roll their leaves under the same field conditions that cause the non genetically engineered plants to roll their leaves. Leaf rolling is a common response of plants such as corn to the water conditions.

Stomata cells. Courtsey of R.L. Neilsen, Purdue University, 2002

Quiz

Question

Question: The reason corn plants will roll their leaves is because rolling reduces the surface of the leaf and this?

To review this concept click on the link: Transpiration - Factors Affecting Rates of Transpiration.

Looks Good! Correct: Thicker boundary layers reduce water loss.
Question

Question: The reason the genetically engineered plants are not rolling their leaves is because?

Looks Good! Correct: Corn plants roll their leaves if they have lost too much water.

Jim’s concern with the genetic engineering approach is that by making the DREB1A gene more easily turned on when stress occurs, this transgene could have a drag on the yield process because the coping mechanisms are also turned on earlier. In other words, the transgenic plant is now more “sensitive” in its response to water stress. The key question then is how soon does the plant begin “saving” water.

Quiz

Question

Question: How can a plant save water?

To review this concept click on the link: Transpiration - What and Why?

Looks Good! Correct: plant can save the water it s currently has by closing stomates because water leaves the plant through the stomates via transpiration.

Jim Specht has read the research reports that indicate that plants that save water also do not yield as well as those plants that use all of the seasonally available water. He has also observed this with in own studies in soybean. He notes that yield is not improved if stomates close sooner and longer, since corn then cannot obtain the carbon dioxide it needs for photosynthesis to fix carbon it needs for vegetative growth and ultimately for grain. . Simply put, the trend in grain crops is that more transpiration – more seed yield (and unfortunately, vice-versa).

Quiz

Question

Question: Open stomates are needed for the corn plant to obtain carbon for growth and grain yield because?

To review this concept click on the link: Transpiration - What and Why?

Looks Good! Correct: Carbon enters a plant while water leaves. Therefore yield costs water.

Copyright 2005

Development of this lesson was supported in part by the Cooperative State Research, Education, & Extension Service, U.S. Dept of Agriculture under Agreement Number PX2003-06237 administered by Cornell University, Virginia Tech and the American Distance Education Consortium (ADEC) and in part by the New Mexico and Nebraska Agricultural Experiment Stations. Any opinions , findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.