Step One: Make Sticky Ends

When a restriction enzyme such as BamHI is used to cut the plasmid, it would cut the circle at one place. The cut would open up the circle in the LacZ gene. This is because gene cloners have placed a piece of DNA that has many restriction enzyme cutting sites within the LacZ gene. This part of the plasmid is called the multicloning site because it gives the gene cloner many options to cut up their DNA and clone it. This is because gene cloners have placed a piece of DNA that has many restriction enzyme cutting sites within the LacZ gene. This part of the plasmid is called the multicloning site because it gives the gene cloner many options to cut up their DNA and clone it. If a sample of DNA cut with the same restriction enzyme was mixed in a tube with the BamHI-cut plasmid, the ends of the DNA molecules could stick together because of their base-pair sequences.